Activities related to Emerging Diseases

Regional Continuing Advanced Hands-on Laboratory Training on Virus Isolation and Identification of PRRS and other Swine Disease Diagnosis

Beijing, P.R. China, 28 November - 2 December 2016

The OIE Regional Representation for Asia and the Pacific (OIE RRAP) has been continuing efforts to control swine diseases in the region, under the support of the Government of People's Republic of China and in collaboration with National Veterinary Diagnostic Laboratory (NVDL; OIE Reference Laboratory for PRRS), China Animal Disease Control Center (CADCC).  In this context, OIE RRAP held Regional Continuing Advanced Hands-on Laboratory Training on Virus Isolation and Identification of PRRS and Other Swine Disease Diagnosis at NVDL located in Beijing, P. R. China on 28 November - 2 December 2016, to which were invited as trainees, 11 experts from Cambodia, P. R. China, Chinese Taipei, Indonesia, Japan, Laos, Mongolia, Myanmar, Philippines, Thailand and Vietnam.  

This is a continuing training of the previous one, Regional Hands-on Laboratory Training on PRRS and Other Swine Disease Diagnosis held at the same laboratory on 13-17 April 2015.  The Objectives of the training were to provide the trainees with opportunities:

1. to enhance their skills in swine viral disease laboratory diagnosis focusing on etiologic diagnosis;

2. to improve the knowledge about establishing new detective method, evaluating and choosing diagnostic reagent kits in clinical application as well as quality control of diagnostic result;

3. to promote further understanding of OIE standards on veterinary laboratory work, including specimen handling and bio-risk management; and

4. to allow experience sharing and to facilitate cooperation between participating countries.

On the first day of the training, after opening remarks and introduction of CADCC and outlines of the training, the trainees were divided into 2 groups, and presented their country reports and discussed them among the groups, and then summarized the reports and their discussion. After then, the trainees learnt cell culture techniques and virus neutralisation test from trainers of NVDL.

On the second day, the trainees learnt the following issues: selection of tests; sample selection; laboratory capacity; quality assurance; and biosafety, as well as major swine diseases including Aujeszky’s disease (AD), CSF, porcine circovirus type-2 (PCVD), porcine epidemic diarrhoea (PED) and swine influenza, and their diagnostic techniques. After then, the trainees practiced virus isolation and culture of PRRS virus as well as TCID50.

On the third day, the trainees were briefed on the situation of PRRS virus in China and its implications for PRRS control. After then, the trainees practiced the fluorescence real-time RT-PCR and got the instruction about Digital RT-PCR.

On the fourth day, the trainees practiced immunofluorescence assay and immunoperoxidase monolayer assay for the detection of PRRS virus and observed cytopathic effect and the results of virus neutralisation tests. In addition, they learnt Digital RT-PCR and analysed its result.

On the morning of the last day, the trainees observed TCID50 and then made a brief discussion about the possibility of establishment of laboratory network in the region. Some trainees proposed as possible activities of the network the following: proficiency test for diagnosis of PRRS, CSF and other diseases; provision of positive samples from OIE Reference Laboratories; establishment of focal points to communicate among laboratories; and every-year conference for experts. On the afternoon of the day, all trainees made brief comments about the training and the certificates of attendance were issued to them.

The OIE is grateful to the Government of People’s Republic of China and to CADCC for giving the trainees the opportunity to learn from basic techniques such as cell culture to state-of-the-art technologies such as Digital RT-PCR.


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